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Pullulanase: High activity enzyme to digest pullulan.
Applications: include: Biofuels, Food Processing, Starch Processing, Brewing, Baking & Flour Cyclodextrin Production.
Properties: Pullulanase is a high-performance starch debranching enzyme designed to unlock maximum value from starch-based processes by selectively cleaving α-1,6 branch points in amylopectin and limit dextrins. By converting branched starch into more linear chains, Pullulanase dramatically boosts the efficiency of downstream enzymes like glucoamylase and β-amylase, delivering higher sugar yields, faster saccharification, and lower residual dextrins—which translates directly into improved throughput, cleaner syrups, and more consistent product quality. Whether you’re producing glucose or high-maltose syrups, optimizing bioethanol yields, or driving higher attenuation in brewing, Pullulanase gives you a simple, cost-effective way to increase conversion, reduce processing time, and improve overall plant performance with minimal process change.
Reaction:
Starch + H2O → Glucan Fragments
- Certification: Food grade, ISO 22000
- Activity: 5,000 U per mL
- Format: Brown liquid
- Temperature Range: 30–65°C
- Optimal Temperature: 45–65°C
- pH Range: 4.5–7.0
- Optimal pH: 5.0–6.5
- Typical Dosage: 0.1–1% w/v
- Storage: 12 months
* Where 1 unit is the amount of enzyme required to release 1 μmol of maltotriose per minute under defined conditions

Supporting technical guides
Starch and fermentation
What Does Pullulanase Do in Starch Saccharification?
Learn when pullulanase can help starch conversion, how debranching complements amylases, and what to measure before adding it to a process.
Read technical guide
Enzyme science · Deep dive
Pullulanase: structure, mechanism and industrial uses
Debranching supports high-glucose or high-maltose syrups and enables more complete use of branched starch dextrins.
Read technical guide